The diazepam-tramadol group had significantly higher blood glucose levels than the naloxone-tramadol group ( p < 0.05). ResultsĪccording to the GEE model results, the diazepam-tramadol and naloxone-diazepam-tramadol groups showed blood glucose levels five units higher than the tramadol group ( p < 0.05). Data were analyzed using SPSS software at a significance level of 0.05 using a one-way analysis of variance (ANOVA) and a generalized estimating equation (GEE). Blood samples were then taken, and the serum obtained was used to verify the fasting glucose concentration. The rats were anesthetized and sacrificed 24 h after the last injection. On the last day, animals’ tail vein blood glucose levels (BGL) were measured using a glucometer at different times, including before the tramadol injection (baseline) and 1 hour, 3 hours, and 6 hours after wards. They received a 75 mg/kg dose of tramadol alone with naloxone, diazepam, and a combination of both of these two drugs. The animals were randomly divided into five groups of 9.

This study was conducted in 45 male Wistar rats. Due to the unknown effects of tramadol poisoning and some of its treatments on blood glucose levels, this study was conducted to investigate the overdose of tramadol and its common treatments (naloxone, diazepam), and their combination on blood glucose levels in male rats.

Various treatments are applied for tramadol poisoning. Tramadol is a synthetic opioid and poisoning is increasing around the world day by day.

Diazepam od antidote